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KMID : 0545120080180121874
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Journal of Microbiology and Biotechnology
2008 Volume.18 No. 12 p.1874 ~ p.1883
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Culture-Independent Analysis of Microbial Succession During Composting of Swine Slurry and Mushroom Cultural Wastes
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Cho Kye-Man
Lee Sun-Mi
Renukaradhya K. Math
Asraful Islam Shah Md.
Kambiranda Devaiah M.
Kim Jong-Min
Yun Myoung-Geun
Cho Ji-Joong
Kim Jong-Ok
Lee Young-Han
Kim Hoon
Yun Han-Dae
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Abstract
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Bacterial diversity and the composition of individual communities during the composting process of swine and mushroom cultural wastes in a field-scale composter (Hazaka system) were examined using a PCR-based approach. The composting process was divided into six stages based on recorded temperature changes. Phylogenetic analysis of eighty 16S rRNA sequences from uncultured composting bacterial groups revealed the presence of representatives from three divisions, including plant pathogenic bacteria, high-molecule-degrading bacteria and spore-forming bacteria. The plant pathogen A. tumefaciens gradually decreased in abundance during the composting process and eventually disappeared during the thermophilic and cooling stage. A bacterium homologous to Bacillus humi first appeared at the early thermophilic stage and was established at the intermediate thermophilic, post-thermophilic, and cooling stages. It was not possible to isolate the B. humi during any of the stages using general culture techniques.
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KEYWORD
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Field-scale composter, swine slurry and mushroom cultural wastes, uncultivable bacterium, bacterial diversity, 16S rRNA gene
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